<i>in vitro</i>翻訳阻害アッセイについての3種の修飾デオキシニバレノールを用いた追加評価

In a previous study, an in vitro cell-free assay of translation inhibition by H-2 toxin, HT-2 and deoxynivalenol using commercially available kit was developed. They showed significant the system. For further evaluation, I examined three additional modified compounds, 3-acetyldeoxynivalenol, 15-acetyldeoxynivalenol, deoxynivalenol-3-glucoside, this study. 15-acetyldeoxynivalenol inhibited as well assay. On other hand, 3-acetyldeoxynivalenol deoxynivalenol-3-glucoside show around 100 times lower no inhibitory activities, respectively, on These data are consistent with studies, suggesting that is useful. It hoped it will be applied to trichothecenes future.